BBa_K431009

BBa_K431009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K431009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: David Joseph Sexton Jr.

Date created: 2010-09-20 11:00:00

Date modified: 2015-05-08 01:12:29

glyceraldehyde 3-phosphate dehydrogenase promoter (pGAP)

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K431009_sequence (Version 1)

Description

This promoter is responsible for the transcription of glyceraldehyde 3-phosphate dehydrogenase in Pichia pastoris. Because this is a key enzyme for glycolysis, it is a strong constitutive promoter. This promoter is one of the most common alternatives to pAOX1 for heterologous protein production in P. pastoris. It is advantageous because its use obviates the hazards and expenses involved with using methanol and still provides high yields of protein.

Notes

The following primers were used to isolate a 0.9kb segment known to contain the pGAP regulatory activity

Forward:5'GCTCTAGGCAGCGAGCTGATCCTTTTTTGTAT
Reverse:5'GGCCACTAGTTGTGTTTTGATAGTTGTTC

Source

This part came from the genome of P. pastoris

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pGAP	1,493	feature/promoter, promoter

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K431009/1

Member of these Collections

• //chassis/eukaryote/pichia
• //promoter
• iGEM Parts Registry