Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Joseph Sexton Jr.
Date created: 2010-09-20 11:00:00
Date modified: 2015-05-08 01:12:29
Formaldehyde Dehydrogenase (FLD1) from Pichia pastoris
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K431010_sequence (Version 1) |
Description
Formaldehyde Dehydrogenase (FLD1) from Pichia pastorisFormaldehyde is a convergent intermediate for utilization of both methanol as a single carbon source and methylamine as a single nitrogen source in Pichia pastoris. Formaldehyde Dehydrogenase is responsible for the oxidation of formaldehyde into formate and is required for cell survival when grown on either methanol or methylamine. For this reason FLD1 has been developed into an alternative marker gene for DNA transformations in fld1- P. pastoris strains.
It has also been shown that cultures with multiple copies of their expression cassette can be obtained by selecting for organisms with increased formaldehyde resistance. This is a similar concept to that used with increased antibiotic resistance but does not pose a biohazard risk. It also avoids multiple transformations, each of which requires a different marker gene.
Users can therefore use this part for selectivity of transformed cells and to obtain high copies of their desired expression cassette.