BBa_K431010

BBa_K431010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K431010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Joseph Sexton Jr.
Date created: 2010-09-20 11:00:00
Date modified: 2015-05-08 01:12:29

Formaldehyde Dehydrogenase (FLD1) from Pichia pastoris



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K431010_sequence (Version 1)

Description

Formaldehyde Dehydrogenase (FLD1) from Pichia pastoris

Formaldehyde is a convergent intermediate for utilization of both methanol as a single carbon source and methylamine as a single nitrogen source in Pichia pastoris. Formaldehyde Dehydrogenase is responsible for the oxidation of formaldehyde into formate and is required for cell survival when grown on either methanol or methylamine. For this reason FLD1 has been developed into an alternative marker gene for DNA transformations in fld1- P. pastoris strains.

It has also been shown that cultures with multiple copies of their expression cassette can be obtained by selecting for organisms with increased formaldehyde resistance. This is a similar concept to that used with increased antibiotic resistance but does not pose a biohazard risk. It also avoids multiple transformations, each of which requires a different marker gene.

Users can therefore use this part for selectivity of transformed cells and to obtain high copies of their desired expression cassette.

Notes

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Source

Pichia pastoris genome

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K431010/1