BBa_K496003

BBa_K496003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K496003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shunichi MAKINO
Date created: 2010-10-16 11:00:00
Date modified: 2015-05-08 01:12:29

RBS + SlrP secretion tag, salmonella T3 sec. sys.



    • Details

    Types
    DnaRegion

    Roles
    tag

    Tag

    Sequences BBa_K496003_sequence (Version 1)

    Description

    leucine-rich repeat protein (SlrP)
    One of the secretion signals in Salmonella Type 3 Secretion System(T3SS). In theory can be used to secrete tagged proteins through Type 3 Secretion Apparatus(T3SA). Due to functional restrictions of T3SA it's known that it can't transport very stable proteins like zinc fingers. Regardless it was shown to transport such stable proteins like GFP.

    Currently this part is in trials to see if it will secrete GFP. Chassis is E.coli with BAC vector fragment (SGSC4024 1464540-1562427) of salmonella SPI2.

    Please consult papers bellow about details
    A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium
    Edward A. Miao and Samuel I. Miller
    2000

    Notes

    RBS was added by primer in the process of PCR.

    Source

    This parts source is from genomic sequence of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2. Amplified b
    y PCR.

    Sequence Annotation Location Component / Role(s)
    BBa_B0034
    SlrP
    		1,12
    15,587
    		engineered_region feature/BioBrick
    feature/tag tag

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K496003/1
     

    Attachments

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