Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shunichi MAKINO
Date created: 2010-10-16 11:00:00
Date modified: 2015-05-08 01:12:29
Reporter of Salmonella secretion signal with NLS, GFP, etc
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K496004_sequence (Version 1) |
Description
This part is for checking if Salmonella Type 3 Secretion Apparatus transplanted to E.coli will work. Please check for more info about SlrP tag - BBa_K496003.Currently performing experiment to see if works.
AraC + RBS + SlrP + NLSx3 + GFP + dT
Notes
SlrP signal source is Salmonella enterica subsp. enterica serovar Typhimurium str. LT2. Amplified b y PCR.Source
SlrP amplified with primer with RBS and flanking restriction site.| Sequence Annotation | Location | Component / Role(s) |
| BBa_I0500 (Arabinose Promoter) BBa_K496003 (RBS and Slrp signal) BBa_J63008 (NLS) BBa_J63008 (NLS) BBa_J63008 (NLS) BBa_E0040 (GFP) BBa_B0015 (dT) | 1,1210 1218,1804 1811,1831 1832,1852 1853,1873 1874,2593 2602,2730 | feature/BioBrick engineered_region engineered_region feature/BioBrick tag feature/tag tag feature/tag tag feature/tag CDS feature/protein feature/BioBrick engineered_region |