Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Caballero & Fernando Govantes
Date created: 2011-09-18 11:00:00
Date modified: 2015-05-08 01:12:30
pUC18R6KT-miniTn7BB-Gm-lacZ+GFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K510018_sequence (Version 1) |
Description
The mini-Tn7-Gm-RBS+RFP artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a lacZ-alfa+GFP-AAV cassette (BBa_I732094) in its BioBrick cloning site (BCS). This plasmid can be used for promoter characterization purposes inserting the promoter using the prefix restriction sites. Also the characterization can be performed in single copy by integrating the device in the genome of the working organism.Notes
The BBa_K093005 BioBrick was inserted within the BCS of pUC18R6KT-miniTn7BB-Gm by EcoRI and PstI clonning.Source
The pUC18R6KT vector was amplified by PCR using as template a pTNS2 plasmid (GenBank accession number: AY884833) provided by Herbert P. Schweizer. The primers were designed to flanked the pUC18R6KT with SfiI restriction sites and allow the insertion of the mini-Tn7-Gm. Primers: attcGGCCTAGGCGGCCgtcgttttacaacgtcgtgac and attcGGCCGCCTAGGCCggaagcataaagtgtaaagcctg. The miniTn7BB-Gm minitransposon was synthesized commercially, and then digested at the flanking SfiI sites and cloned into SfiI-digested pUC18R6KT PCR products.| Access | Instance | Definition |
| public public | BBa_K510012 BBa_I732094 | BBa_K510012 BBa_I732094 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K510012 BBa_I732094 | 1,4549 4558,5758 | BBa_K510012 BBa_I732094 |