BBa_K510022

BBa_K510022 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K510022
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Caballero
Date created: 2011-09-18 11:00:00
Date modified: 2015-05-08 01:12:30

attTn7



    • Details

    Types
    DnaRegion

    Roles
    Other

    sequence_feature

    Sequences BBa_K510022_sequence (Version 1)

    Description

    TnsABC+D transposase machinery causes the site-specific insertion of the Tn7 transposon in the attTn7 (from attachment site) sequence, which is found in many bacteria. This insertion site is well conserved in Gram-negative bacteria, an even in higher organisms until humans. However, here we add a portable attTn7 to allow using the miniTn7 BioBrick toolkit in any organism as well as in plasmids.
    All the derivatives of the miniTn7 BioBrick toolkit could be inserted in the attTn7 site by transposition.

    Notes

    The attTn7 site was flanked by prefix and suffix for cloning purposes.

    Source

    The attTn7 was obtained from the purified genome of E. coli MC4100 by PCR (using a high accuracy Pfu polymerase) with these primers: gaattcgcggccgcttctagagTGCAGCTGCTGGCTTACCATG and ctgcagcggccgctactagtaACATGGAGTTGGCAGGATGTTTG.

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K510022/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, and collaborators
    About SynBioHub | View Source on Github | Report an Issue
    | v1.6.1 (57f00336)