Types | DnaRegion
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Roles | Other
sequence_feature
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Sequences | BBa_K510022_sequence (Version 1)
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Description
TnsABC+D transposase machinery causes the site-specific insertion of the Tn7 transposon in the attTn7 (from attachment site) sequence, which is found in many bacteria. This insertion site is well conserved in Gram-negative bacteria, an even in higher organisms until humans. However, here we add a portable attTn7 to allow using the miniTn7 BioBrick toolkit in any organism as well as in plasmids.
All the derivatives of the miniTn7 BioBrick toolkit could be inserted in the attTn7 site by transposition.
Notes
The attTn7 site was flanked by prefix and suffix for cloning purposes.
Source
The attTn7 was obtained from the purified genome of E. coli MC4100 by PCR (using a high accuracy Pfu polymerase) with these primers: gaattcgcggccgcttctagagTGCAGCTGCTGGCTTACCATG and ctgcagcggccgctactagtaACATGGAGTTGGCAGGATGTTTG.