BBa_K510022

BBa_K510022 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K510022
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Caballero
Date created: 2011-09-18 11:00:00
Date modified: 2015-05-08 01:12:30

attTn7



Types
DnaRegion

Roles
Other

sequence_feature

Sequences BBa_K510022_sequence (Version 1)

Description

TnsABC+D transposase machinery causes the site-specific insertion of the Tn7 transposon in the attTn7 (from attachment site) sequence, which is found in many bacteria. This insertion site is well conserved in Gram-negative bacteria, an even in higher organisms until humans. However, here we add a portable attTn7 to allow using the miniTn7 BioBrick toolkit in any organism as well as in plasmids.
All the derivatives of the miniTn7 BioBrick toolkit could be inserted in the attTn7 site by transposition.

Notes

The attTn7 site was flanked by prefix and suffix for cloning purposes.

Source

The attTn7 was obtained from the purified genome of E. coli MC4100 by PCR (using a high accuracy Pfu polymerase) with these primers: gaattcgcggccgcttctagagTGCAGCTGCTGGCTTACCATG and ctgcagcggccgctactagtaACATGGAGTTGGCAGGATGTTTG.

igem#experience
Works
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K510022/1