| Types | DnaRegion
|
| Roles | Composite
engineered_region
|
| Sequences | BBa_K510030_sequence (Version 1)
|
Description
It is composed of two parts. The first one is the ADH1 terminator, which functions as an insulator or boundary for transcription isolation. The second one is formed by two tetR operator sequences (tetO2), of 19 nucleotides each one, alternated with spacers. This composite part should be inserted just in front of a promoter in order to control its regulations by generating a nucleated structure of heterochromatinic DNA, together with his associated part tetO4-terminator. Compaction of DNA is due to binding of engineered silencing proteins (tetR+heterochromatin forming protein)to tetO sequences.
