BBa_K510031

BBa_K510031 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K510031

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Paola Gallardo, Rafael R. Daga

Date created: 2011-09-19 11:00:00

Date modified: 2015-05-08 01:12:30

GFP-Tadh1- tetO4- Tact1

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K510031_sequence (Version 1)

Description

This composite part is formed by four main elements. Coding sequence of GFP reporter protein, followed by the transcriptional terminator of ADH1 gene. The next part is denominated tetO4, that consist of four 19 nucleotices tetR operator sequences, alternated with spacers. And the last part is the Act1 transcriptional terminator that function as insulator. This part should be interted behind a promoter. In association with Tadh1-tetO2 part, it functions as a transcription regulator, by compacting intermediate sequence.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
GFP	1,726	CDS, feature/cds
adh1 terminator	735,914	stem_loop, feature/stem_loop
tetO	952,970	operator, feature/operator
tetO	994,1012	operator, feature/operator
tetO	1036,1054	operator, feature/operator
tetO	1078,1096	feature/operator, operator
act1 terminator	1120,1261	feature/stem_loop, stem_loop

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K510031/1

Member of these Collections

• iGEM Parts Registry