BBa_K510039

BBa_K510039 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K510039
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Caballero, Fernando Govantes
Date created: 2011-10-18 11:00:00
Date modified: 2015-05-08 01:12:30

pUC18Sfi-miniTn7BB-Gm-tetR



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K510039_sequence (Version 1)

Description

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Notes

The BBa_I732083 BioBrick was inserted within the BCS of pUC18Sfi-miniTn7BB-Gm by EcoRI and PstI clonning.
In order to construct the pUC18Sfi-miniTn7BB-Gm delivery plasmid, the miniTn7BB-Gm transposon was cleaved from the commercial plasmid pMA (Mr. Gene) with SfiI and ligated to SfiI-digested pUC18Sfi. This strategy removes all multi-cloning restriction sites from pUC18Sfi, except for the flanking SfiI, thus guaranteeing that the unique sites in the transposon are not duplicated. The remove of the multi-cloning restriction sites was verify by analytic digestions.

Source

The miniTn7BB-Gm minitransposon was synthesized commercially and pUC18SfiI is a commercial vector.

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K510039/1