Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Caballero
Date created: 2011-10-20 11:00:00
Date modified: 2015-05-08 01:12:30
pUC18Sfi-miniTn7BB-Gm-bistable_switch
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K510043_sequence (Version 1) |
Description
This plasmid allows the integration of a transcriptional flip-flop into bacterial chromosomes using the miniTn7BB characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.Notes
The BBa_K177038 BioBrick was inserted within the BCS of pUC18Sfi-miniTn7BB-Gm by EcoRI and PstI clonning.In order to construct the pUC18Sfi-miniTn7BB-Gm delivery plasmid, the miniTn7BB-Gm transposon was cleaved from the commercial plasmid pMA (Mr. Gene) with SfiI and ligated to SfiI-digested pUC18Sfi. This strategy removes all multi-cloning restriction sites from pUC18Sfi, except for the flanking SfiI, thus guaranteeing that the unique sites in the transposon are not duplicated. The remove of the multi-cloning restriction sites was verified by analytic digestions.
Source
The miniTn7BB-Gm minitransposon was synthesized commercially and pUC18SfiI is a commercial vector.| Access | Instance | Definition |
| public public | BBa_K510000 BBa_K177038 | BBa_K510000 BBa_K177038 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K510000 BBa_K177038 | 1,4388 4397,8329 | BBa_K510000 BBa_K177038 |