Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-24 11:00:00
Date modified: 2015-05-08 01:12:31
minCMV-1xCI434 Promoter MammoBlock
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K511001_sequence (Version 1) |
Description
This part encodes a promoter that is moderately inducible by the CI434-VP16 transactivator and constitutively off otherwise.As shown in Figures 1 and 2 below, induction with the CI434-VP16 transactivator results in marked increase in fluorescence from a construct containing a red fluorescent protein (mKate) driven by the minCMV-1xCI434 promoter.
Notes
This particular promoter has a propensity to mutate in cells that are not exceptionally recombination-deficient, and caution should be taken in producing both entry and expression vectors using this part.Source
This promoter is a synthetic construct composed of the minimal sequence required for transcription from the immediate-early cytomegalovirus (CMV) promoter preceded by a binding site for the CI434 protein adapted from bacteriophage 434.| Sequence Annotation | Location | Component / Role(s) |
| CI434 Box minCMV Promoter | 1,46 56,113 | feature/operator operator feature/promoter promoter |