BBa_K511003

BBa_K511003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K511003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grant Robinson

Date created: 2011-09-24 11:00:00

Date modified: 2015-05-08 01:12:31

UAS-Gal4 Promoter MammoBlock

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K511003_sequence (Version 1)

Description

This part encodes a promoter that is inducible by variants of the Gal4 transactivator and off otherwise.

As shown in Figures 1 and 2, fluorescence from a red fluorescent protein (mKate) driven by the UAS-Gal4 promoter is very strong in the presence of constitutively produced Gal4 derivative with an attached viral VP16 transactivation domain (Gal4-VP16), and is essentially off in the absences of the Gal4-VP16 transactivator.

Notes

Not applicable.

Source

This part was synthesized to include the upstream activator sequence (UAS) from the Saccharomyces cerevisiae genome that binds to the Gal4 transactivator upstream of the major-late adenovirus promoter.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Gal4-Binding Region	46,148	operator, feature/operator
Major-Late Adenovirus Promoter	160,200	promoter, feature/promoter

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K511003/1

Member of these Collections

• iGEM Parts Registry