BBa_K511004

BBa_K511004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K511004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grant Robinson

Date created: 2011-09-27 11:00:00

Date modified: 2015-05-08 01:12:31

TRE-Tight-LacOid Promoter MammoBlock

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K511004_sequence (Version 1)

Description

This part encodes an inducible promoter that can be activated by tTA/rtTA transactivator variants in the presence of tetracycline analogues and can be repressed by variants of the LacI transcriptional repressor.

Notes

Not applicable.

Source

This part is a synthetic promoter formed from the minimal transcriptional sequence necessary for the immediate-early cytomegalovirus (CMV) promoter, a LacOid operator that has been computationally shown to bind to the LacI repressor protein more tightly than the wildtype LacO operator, and six tetracycline response elements (TREs) isolated from Escherichia coli.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
TRE-LacO Promoter	1,338	promoter, feature/promoter

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K511004/1

Member of these Collections

• iGEM Parts Registry