Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-24 11:00:00
Date modified: 2015-05-08 01:12:31
EYFP-FF4x4 MammoBlock
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K511101_sequence (Version 1) |
Description
This part is an engineered variant of the original green fluorescent protein from Aequorea victoria that fluoresces yellow. Additionally, this protein has four binding sites for the FF4 micro RNA (miRNA). In the presence of FF4 miRNA, translation of this protein is markedly attenuated.Figures 1 and 2 show typical levels of EYFP-FF4x4-mediated fluorescence following transfection of HEK-293 cells with EYFP-FF4x4 driven by the Hef1a-LacOid low-level constitutive promoter.
Notes
The presence of FF4 binding sites on this protein will preclude its use in certain cells that naturally produce the FF4 miRNA, and it is recommended that parties interested in using this part consult references explaining which miRNAs are produced in their desired cell types.Source
This protein is an engineered version of the original green fluorescent protein from Aequorea victoria that has ben modified to fluoresce yellow.| Sequence Annotation | Location | Component / Role(s) |
| Enhanced Yellow Fluorescent Protein Four FF4 Binding Sites | 1,720 732,819 | feature/cds CDS operator feature/operator |