Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-24 11:00:00
Date modified: 2015-05-08 01:12:31
rtTA-DD Transactivator MammoBlock
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K511202_sequence (Version 1) |
Description
This part encodes a protein that combines the rtTA transactivator with the DD destabilization domain. The DD domain causes attached proteins to be degraded extremely quickly in mammalian cells; however, the DD domain is stable in the presence of the SHIELD molecule, allowing for tunable protein expression. Because the rtTA transactivator is dependent upon doxycycline for activation, transactivation by rtTA-DD is unique among transactivators for the dynamic range of expression it can activate.Notes
Note that the DD domain is positioned before the rtTA transactivator in this part.Source
This part consists of an Escherichia coli-derived binding domain for the tetracycline operator, the viral VP16 transactivation domain isolated from the herpes simplex virus, and an engineered human FKBP12 protein that functions as a destabilization domain.| Sequence Annotation | Location | Component / Role(s) |
| Destabilization Domain rtTA Transactivation Domain | 1,324 325,1329 | tag feature/tag CDS feature/protein |