BBa_K511202

BBa_K511202 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K511202

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grant Robinson

Date created: 2011-09-24 11:00:00

Date modified: 2015-05-08 01:12:31

rtTA-DD Transactivator MammoBlock

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K511202_sequence (Version 1)

Description

This part encodes a protein that combines the rtTA transactivator with the DD destabilization domain. The DD domain causes attached proteins to be degraded extremely quickly in mammalian cells; however, the DD domain is stable in the presence of the SHIELD molecule, allowing for tunable protein expression. Because the rtTA transactivator is dependent upon doxycycline for activation, transactivation by rtTA-DD is unique among transactivators for the dynamic range of expression it can activate.

Notes

Note that the DD domain is positioned before the rtTA transactivator in this part.

Source

This part consists of an Escherichia coli-derived binding domain for the tetracycline operator, the viral VP16 transactivation domain isolated from the herpes simplex virus, and an engineered human FKBP12 protein that functions as a destabilization domain.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Destabilization Domain	1,324	tag, feature/tag
rtTA Transactivation Domain	325,1329	CDS, feature/protein

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K511202/1

Member of these Collections

• iGEM Parts Registry