Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:31
HRH4-TEVs-GV16 Signaling Receptor MammoBlock
| Types | DnaRegion |
| Roles | engineered_region Signalling |
| Sequences | BBa_K511401_sequence (Version 1) |
Description
This part encodes a fusion protein consisting of the human HRH4 receptor, the recognition sequence for the tobacco etch virus (TEV) protease, and the Gal4-VP16 transactivator.Notes
The TEV recognition sequence encoded in the rough center of this part is flanked on both sides by sequences that code for glycine-serine-glycine linkers. This feature was designed to serve as a homologous region allowing for facile substitution of either a receptor or a transactivator around the TEV recognition sequence using the Gibson ligation assembly method.Source
This part includes human histamine receptor 4 (HRH4), the tobacco etch virus (TEV) protease recognition sequence, the DNA-binding domain from the Gal4 transactivator of Saccharomyces cerevisiae, and the VP16 transactivation domain from the herpes simplex virus.| Sequence Annotation | Location | Component / Role(s) |
| HRH4 Receptor Flexible TEV Recognition Sequence Gal4-VP16 Transactivator | 1,1177 1177,1216 1217,1896 | feature/protein CDS tag feature/tag CDS feature/protein |