Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:31
N-Cadherin-2A-EYFP Cell Adhesion MammoBlock
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K511600_sequence (Version 1) |
Description
This part encodes a protein kmown to be instrumental in intercellular adhesion and tissue integrity. Like all cadherins, N-Cadherin mediates adhesion with five extracellular, calcium-binding repeats and an intercellular domain that makes contacts with the actin cytoskeleton of mammalian cells. This part additionally encodes an enhanced yellow fluorescent protein (EYFP) linked to the N-Cadherin by a self-cleaving viral 2A peptide, allowing for bicistronic expression of both a cell adhesion protein and an identifying marker with a single part.Notes
Not applicable.Source
The N-Cadherin sequence of this part was isolated from a murine source; the viral 2A self-cleaving peptide was isolated from a virus with homology to the foot-and-mouth disease virus; the EYFP fluorescent reporter is an engineered version of the original green fluorescent protein from Aequorea victoria.| Sequence Annotation | Location | Component / Role(s) |
| Murine N-Cadherin 2A Self-Cleaving Peptide EYFP | 1,2718 2719,2794 2719,3511 | CDS feature/protein tag feature/tag CDS feature/protein |