Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:31
B-Arrestin-2-TEV-Protease MammoBlock
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K511701_sequence (Version 1) |
Description
This part encodes a fusion between the N1a catalytic proteolytic domain of the tobacco etch virus (TEV) and the human B-Arrestin-2 sequence. The N1a domain recognizes the amino acid sequence ENLYFQX (where X is a variable amino acid) with unparalleled specificity, and is typically used for the removal of tags made for protein purification. The B-Arrestin-2 protein induces the extinction of nonvisual G-coupled protein receptor (GPCR) signaling in mammalian cells by binding to the cytoplasmic tails of induced GPCRs. This part is capable of cleaving GPCR-TEVs-Transactivator fusion proteins and freeing the tethered transactivator to induce transcriptional changes in a cell's nucleus.Notes
Not applicable.Source
The N1a domain was isolated from the tobacco etch mosaic virus while the B-Arrestin-2 protein was isolated from a human cDNA library.| Sequence Annotation | Location | Component / Role(s) |
| B-Arrestin-2 Double-Glycine Linker TEV Protease | 1,1233 1234,1239 1240,1890 | feature/protein CDS tag feature/tag CDS feature/protein |