Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:31
Mnt-VP16-Inducible Red Fluorescence Generator (minCMV-4xMnt-mKate) MammoBlock Device
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K511813_sequence (Version 1) |
Description
This MammoBlock composite device produces the monomeric red fluorescent protein mKate driven by the minCMV-4xMnt promoter. In the absence of Mnt-VP16, transcription of mKate is very low, but in the presence of CI434-VP16, transcription is moderate.Notes
In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.Source
Consult constituent components.| Access | Instance | Definition |
| public public public | BBa_K511002 BBa_J85600 BBa_K511102 | BBa_K511002 BBa_J85600 BBa_K511102 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K511002 BBa_J85600 BBa_K511102 | 1,347 348,369 370,1083 | BBa_K511002 BBa_J85600 BBa_K511102 |