BBa_K511813

BBa_K511813 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K511813
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:31

Mnt-VP16-Inducible Red Fluorescence Generator (minCMV-4xMnt-mKate) MammoBlock Device



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K511813_sequence (Version 1)

Description

This MammoBlock composite device produces the monomeric red fluorescent protein mKate driven by the minCMV-4xMnt promoter. In the absence of Mnt-VP16, transcription of mKate is very low, but in the presence of CI434-VP16, transcription is moderate.

Notes

In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.

Source

Consult constituent components.

igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K511813/1