Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:31
Inducible Yellow Fluorescent Protein Generator (UAS-Gal4-Citrine) MammoBlock Device
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K511819_sequence (Version 1) |
Description
This MammoBlock composite device produces the yellow/green fluorescent protein Citrine when induced with Gal4 transactivator variants and otherwise produces Citrine at a low, basal (OFF) level.Notes
In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors. As an additional note for this part, we made use of an expression vector generated by the Elowitz lab in our initial experiments, and did not convert the Citrine sequence into an entry vector format.Source
Consult constituent components.| Access | Instance | Definition |
| public public public | BBa_K511003 BBa_J85600 BBa_J18931 | BBa_K511003 BBa_J85600 mCitrine |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K511003 BBa_J85600 BBa_J18931 | 1,220 221,242 243,956 | BBa_K511003 BBa_J85600 mCitrine |