Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Arcanum
Date created: 2011-09-18 11:00:00
Date modified: 2015-05-08 01:12:32
C6-3OXO-HSL -> β
-galactosidase activity
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K514000_sequence (Version 1) |
Description
This BioBrick is a composition of F2620 with I732019. It was conceived to characterize the construction F2620: check which concentration of its specific quorum sensing signal (C6-3-oxo-HSL) it's able to detect, and how long does it take the bacterium to produce an answer (in this case, the expression of lacZ).Notes
It has been very difficult to join F2620 with I142019 by the 3A method. In fact, it has been difficult for all BioBricks. Maybe it's a good method for a machine, because you don't have to purify from electrophoresis gel, but DNA concentrations suffer a great decay in the process. Use the standard method.Source
Parts distribution of 2011.| Access | Instance | Definition |
| public public | BBa_F2620 BBa_I732019 | BBa_F2620 BBa_I732019 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_F2620 BBa_I732019 | 1,1061 1070,4299 | BBa_F2620 BBa_I732019 |