BBa_K518002

BBa_K518002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K518002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Masato Ohgishi

Date created: 2011-09-14 11:00:00

Date modified: 2015-05-08 01:12:33

Firefly-renilla dual luciferase assay kit

Details

• Types: DnaRegion
• Roles: Measurement; engineered_region
• Sequences: BBa_K518002_sequence (Version 1)

Description

Luciferase assay is one of the most popular reporter assay system for quantitatively measuring the function of promoters and other cis-elements. The wide-ranging and quantitative detection is the prominent feature of this assay; light emmision from 10~(-18) ― 10~(-11) mol protein can be quantitatively measured using luminometer (see our results).
A promoter or other cis-element to be analysed can be ligated above this part. Its effect will be measured as an altered firefly luciferase expression. Meanwhile, renilla luciferase expression can be used to as an internal control.

Notes

None

Source

From BioBrick parts.

Components

Access	Instance	Definition
public	BBa_B0032	BBa_B0032
public	BBa_I712019	BBa_I712019
public	BBa_B0014	BBa_B0014
public	BBa_J23118	BBa_J23118
public	BBa_B0032	BBa_B0032
public	BBa_J52008	BBa_J52008
public	BBa_B0014	BBa_B0014

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0032	1,13	BBa_B0032
BBa_I712019	20,1672	BBa_I712019
BBa_B0014	1681,1775	BBa_B0014
BBa_J23118	1784,1818	BBa_J23118
BBa_B0032	1827,1839	BBa_B0032
BBa_J52008	1846,2781	BBa_J52008
BBa_B0014	2790,2884	BBa_B0014

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K518002/1

Member of these Collections

• //classic/measurement/uncategorized
• //function/reporter/light
• iGEM Parts Registry