Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mun Ching Lee, Sylvia Ispasanie, Allan Crossman
Date created: 2011-07-18 11:00:00
Date modified: 2015-05-08 01:12:33
RBS + malS (E. coli periplasmic α
-amylase)
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K523001_sequence (Version 1) |
Description
This is the E. coli Amylase gene malS. The part contains the native Ribosome Binding Site.The part was made using the strategy outlined in
Notes
The part was made using the strategy outlined inThe part was created from a lab E. coli strain using primers:
*Forward: ACT AGATCT gaa atc gca gca ata agg act c
**adds BglII site; starts upstream of gene to catch RBS; RFC10 compliant after replacement of
*Reverse: ACT ACTAGT A TTA tta ctg ttg ccc tgc cca g
**adds SpeI site; RFC10 compliant after insertion into vector; has double stop codon.
Source
The expected sequence can be seen using GenBank U00096.2| Sequence Annotation | Location | Component / Role(s) |
| BglII Native RBS ATG malS TAATAA Predicted (cleaved) signal peptide | 1,4 20,23 34,34 34,2067 2062,2062 34,84 | sequence_feature feature/misc ribosome_entry_site feature/rbs start_codon feature/start CDS feature/cds stop_codon feature/stop sequence_feature feature/misc |