Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sylvia Ispasanie, Mun Ching Lee
Date created: 2011-07-28 11:00:00
Date modified: 2015-05-08 01:12:33
bglX for BioSandwich
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K523004_sequence (Version 1) |
Description
This is a version ofThere are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site.
There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine.
The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for Edinburgh 2011's BioSandwich assembly protocol.
Notes
The part was made using the strategy outlined inThe part was created from a lab E. coli strain using primers:
- ACT AGATCT atg aaa tgg cta tgt tca gta gg
- Forward; adds BglII site; starts at start codon.
- ACT ACTAGT ACC cag caa ctc aaa ctc gcc ttt c
- Reverse; adds SpeI site; BioSandwich compliant; no stop codons, adds a glycine.