BBa_K523004

BBa_K523004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K523004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sylvia Ispasanie, Mun Ching Lee
Date created: 2011-07-28 11:00:00
Date modified: 2015-05-08 01:12:33

bglX for BioSandwich



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K523004_sequence (Version 1)

Description

This is a version of BBa_K523002 but with no RBS and no stop codon.

There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site.

There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine.

The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for Edinburgh 2011's BioSandwich assembly protocol.

Notes

The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.

The part was created from a lab E. coli strain using primers:

  • ACT AGATCT atg aaa tgg cta tgt tca gta gg
    • Forward; adds BglII site; starts at start codon.


  • ACT ACTAGT ACC cag caa ctc aaa ctc gcc ttt c
    • Reverse; adds SpeI site; BioSandwich compliant; no stop codons, adds a glycine.


Source

See BBa_K523002

Sequence Annotation Location Component / Role(s)
bglX
ATG
GG
BglII
PstI site removed
5,2299
5,5
2300,2301
1,4
52,52
feature/cds CDS
start_codon feature/start
feature/misc sequence_feature
sequence_feature feature/misc
point_mutation feature/s_mutation
igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K523004/1