BBa_K523019

BBa_K523019 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K523019

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sylvia Ispasanie, Mun Ching Lee

Date created: 2011-09-14 11:00:00

Date modified: 2015-05-08 01:12:33

Plac + INP-cex

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K523019_sequence (Version 1)

Description

This is a fusion of Ice Nucleation Protein and the cex exoglucanase, under the control of the lac promoter.

Notes

Assembly from other parts using the BioSandwich protocol of Edinburgh 2011.

Source

Assembly from other parts using the BioSandwich protocol of Edinburgh 2011.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Spacer	1,32	sequence_feature, feature/misc
Plac - LacZ	38,589	sequence_feature, feature/dna
LacZ	359,589	CDS, feature/cds
Spacer	597,625	sequence_feature, feature/misc
rbs	611,614	ribosome_entry_site, feature/rbs
INP-cex fusion	623,2958	CDS, feature/cds
TAA	2951,2951	feature/stop, stop_codon
Spacer	2942,2969	sequence_feature, feature/misc
ATG	623,623	start_codon, feature/start
-35	286,291	promoter, feature/promoter
-10	309,314	feature/promoter, promoter
rbs	348,351	ribosome_entry_site, feature/rbs
Spacer (Linker)	1565,1594	sequence_feature, feature/misc

Other Properties

• igem#experience: Issues
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K523019/1

Member of these Collections

• iGEM Parts Registry