BBa_K523023

BBa_K523023 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K523023
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sylvia Ispasanie, Mun Ching Lee
Date created: 2011-09-14 11:00:00
Date modified: 2015-05-08 01:12:34

PlacLacZ + EYFP



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K523023_sequence (Version 1)

Description

This is the lac promoter driving Enhanced Yellow Fluorescent Protein. It was made as a test of the BioSandwich protocol of Edinburgh 2011.

Notes

N/A.

Source

BioSandwich Assembly of K523000 and K523012

Sequence Annotation Location Component / Role(s)
Spacer
Plac - LacZ
-35
-10
LacZ
GCA to GTA
Spacer
rbs
EYFP
TAATAA
rbs
1,32
38,589
286,291
309,314
359,589
447,447
597,625
611,614
623,1345
1340,1340
348,351
sequence_feature feature/misc
sequence_feature feature/dna
promoter feature/promoter
promoter feature/promoter
CDS feature/cds
feature/mutation sequence_alteration
feature/misc sequence_feature
feature/rbs ribosome_entry_site
CDS feature/cds
feature/stop stop_codon
ribosome_entry_site feature/rbs
igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K523023/1