BBa_K523023

BBa_K523023 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K523023
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sylvia Ispasanie, Mun Ching Lee
Date created: 2011-09-14 11:00:00
Date modified: 2015-05-08 01:12:34

PlacLacZ + EYFP



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_K523023_sequence (Version 1)

    Description

    This is the lac promoter driving Enhanced Yellow Fluorescent Protein. It was made as a test of the BioSandwich protocol of Edinburgh 2011.

    Notes

    N/A.

    Source

    BioSandwich Assembly of K523000 and K523012

    Sequence Annotation Location Component / Role(s)
    Spacer
    Plac - LacZ
    -35
    -10
    LacZ
    GCA to GTA
    Spacer
    rbs
    EYFP
    TAATAA
    rbs
    		1,32
    38,589
    286,291
    309,314
    359,589
    447,447
    597,625
    611,614
    623,1345
    1340,1340
    348,351
    		sequence_feature feature/misc
    sequence_feature feature/dna
    promoter feature/promoter
    promoter feature/promoter
    CDS feature/cds
    feature/mutation sequence_alteration
    feature/misc sequence_feature
    feature/rbs ribosome_entry_site
    CDS feature/cds
    feature/stop stop_codon
    ribosome_entry_site feature/rbs

    igem#experience
    None
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K523023/1
     

    Attachments

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