Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: HO Yuan Heng Trevor
Date created: 2011-10-02 11:00:00
Date modified: 2015-05-08 01:12:34
pir gene
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K524004_sequence (Version 1) |
Description
ori-gamma is part of the replication origin of the R6K origin. Initiation of replication at ori-gamma requires the pi protein in trans, which is encoded by the pir gene. Yet doubling the concentration of pi protein would effectively shut down the replication as well. Expression of pi protein is auto-regulated at its promoter region. Our construct design was to insert a CAP-binding site between the auto-regulating promoter and the CDS, by doing so we can halt replication and induce plasmid loss in nutrient (glucose) rich medium.Notes
We modified the oriniginal pir gene from BL21 by inserting a CAP-binding site between the auto-regulating promoter and the CDSSource
from the gDNA of E.coli strain BL21| Sequence Annotation | Location | Component / Role(s) |
| regulatory binding site for pi protein autogenously regulated promoter pir gene start codon stop codon | 1,57 24,90 91,1008 91,93 1006,1008 | non_covalent_binding_site feature/binding feature/promoter promoter CDS feature/cds start_codon feature/start feature/stop stop_codon |