Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: HO Yuan Heng Trevor
Date created: 2012-08-25 11:00:00
Date modified: 2015-05-08 01:12:34
pSC101 heat sensitive origin of replication with deletion in par locus
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K524203_sequence (Version 1) |
Description
A derivative of pSC101 origin of replication, this heat sensitive origin has the par locus deleted, therefore allowing a rapid loss of plasmid even under the permissive temperature.Notes
The par locus was deleted through excision of BBa_K524000 by SspI and StuI, purification of desired fragments and thenSource
Modified from BBa_K524000| Sequence Annotation | Location | Component / Role(s) |
| repA101ts Inverted Repeat 2 (IR2) -> Inverted Repeat 2 (IR2) <- -10 promoter region of repAts -35 promoter region of repAts Inverted Repeat 1 (IR1) -> Inverted Repeat 1 (IR1) <- Direct Repeat 3 (DR3) Direct Repeat 2 (DR2) Direct Repeat 1 (DR1) IHF binding sequence AT Rich Region DnaD protein binding sequence scar of removed par locus | 373,1323 1325,1348 1349,1371 1354,1359 1376,1381 1371,1384 1385,1397 1420,1441 1453,1473 1474,1494 1514,1526 1492,1547 1582,1590 1734,1739 | CDS feature/cds feature/operator operator feature/operator operator feature/promoter promoter promoter feature/promoter feature/operator operator feature/operator operator feature/operator operator feature/operator operator feature/operator operator feature/binding non_covalent_binding_site operator feature/operator feature/binding non_covalent_binding_site feature/dna sequence_feature |