Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Manuel Wittchen
Date created: 2011-10-24 11:00:00
Date modified: 2015-05-08 01:12:34
Fusion Protein of S-Layer SgsE and LigA with His-Tag
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K525009_sequence (Version 1) |
Description
Fusion Protein of S-Layer SgsE and LigA with His-TagNotes
This part was assembled by Freiburg Standard Assembly (RFC 25)Source
SgsE was synthesized, LigA with His-Tag created by PCR. LigA is the natural ligase A of E. coli.| Sequence Annotation | Location | Component / Role(s) |
| T7 promotor K525303 lac operator B0034 ATG SgsE domain NogMIV Freiburg scar K525740 LigA domain His-Tag | 1,20 1,2409 20,44 53,64 73,75 73,2409 76,82 2401,2407 2410,4434 2407,4416 4417,4434 | feature/promoter promoter engineered_region feature/BioBrick feature/operator operator feature/rbs ribosome_entry_site feature/start start_codon feature/protein CDS feature/binding non_covalent_binding_site sequence_feature feature/misc engineered_region feature/BioBrick CDS feature/protein feature/tag tag |