BBa_K526001

BBa_K526001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K526001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sung Kuk Lee

Date created: 2011-09-25 11:00:00

Date modified: 2015-05-08 01:12:35

ptrc*-Dpn

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K526001_sequence (Version 1)

Description

ptrc* is a modified form of ptrc promoter with the -35 and -10 regions being present in the reverse order and the promoter is flanked by fimE recognition sequence. FimE is an invertase capable of inverting any DNA sequence flanked between its recognition seqence. This fim inversion system would help avoid leaky expression. We expressed a new lysis device, DpnI under the control of fim inversion promoter. DpnI is a restriction enzyme capable of chopping up all methylated DNAs. We propose DpnI as a novel lysis device.
DpnC and DpnD are obtained from Streptococcus pneumoniae R6 strain.

Notes

One should be careful while expressing Dpn genes. Dpn is supposed to chop up the DNA and cause cell death. Hence, no leaky level of Dpn is needed to recover live cells

Source

http://www.ncbi.nlm.nih.gov/nuccore/25307955?from=1637214&to=1637675

http://www.ncbi.nlm.nih.gov/nuccore/25307955?from=1637672&to=1638436

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
DpnC	221,1046	CDS, feature/protein
fim inversion promoter	1,220	promoter, feature/promoter
DpnD	1077,1536	feature/protein, CDS

Other Properties

• igem#experience: Issues
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K526001/1

Member of these Collections

• //biosafety/xnase
• iGEM Parts Registry