Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sung Kuk Lee
Date created: 2011-09-25 11:00:00
Date modified: 2015-05-08 01:12:35
Temperature dependent Ribo-Switch fused to GFP
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K526002_sequence (Version 1) |
Description
We have exploited the temperature dependent mRNA that is associated with the chemotaxis response regulator, CheR, to develop a novel temperature regulated gene expression system. The mRNA is assumed to form a stable secondary structure burrying the RBS and thus blocking gene expression at a higher temperature (37 degree C) where as the temperature is lowered to about 27 degree C the stability of the secondary structure is disturbed thus exposing the RBS and favoring gene expressionNotes
We removed the start codon and the second codon present in the mRNA and fused it directly to GFP. The removal of the last two codons of the mRNA might have some effect on the secondary structure stabilitySource
Oleksiuk, O., Jakovljevic, V., Vladimirov, N., Carvalho, R., Paster, E., Ryu, William??S., Meir, Y., Wingreen, Ned??S., Kollmann, M. and Sourjik, V. Thermal Robustness of Signaling in Bacterial Chemotaxis. Cell 145 (2011), pp. 312-321.| Sequence Annotation | Location | Component / Role(s) |
| Riboswitch GFP | 1,110 111,807 | feature/misc sequence_feature feature/protein CDS |