BBa_K527000

BBa_K527000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K527000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eva Meier Carlsen
Date created: 2011-09-16 11:00:00
Date modified: 2015-05-08 01:12:35

Biosynthesis/Oximes/CYP79A2



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K527000_sequence (Version 1)

Description

This BioBrick BBa_K527000 encode the plant enzyme Cytochrome P450 CYP79A2. Cytochromes p450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation.

CYP79A2 is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of the cyanogenic glycoside benzylglucosinolate. Oximes as the phenylacetaldoxime is generally toxic to fungi and is frequently used as chemical fungicides. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species.

Notes

Mutation at a Pst1 restriction site (nucleotide 343).

Source

Institute of Plant Biology and Biotechnology
Faaculty og LIFE Science
Copenhagen University
Denmark

Sequence Annotation Location Component / Role(s)
start
mutation
stop
Cyp79A2
1,3
342,342
1570,1572
1,1572
feature/start start_codon
sequence_alteration feature/mutation
stop_codon feature/stop
sequence_feature feature/misc
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K527000/1