BBa_K527001

BBa_K527001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K527001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eva Meier Carlsen
Date created: 2011-09-16 11:00:00
Date modified: 2015-05-08 01:12:35

Biosynthesis/Oximes/CYP79A2/Plug 'n' Play



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K527001_sequence (Version 1)

Description

This BioBrick BBa_K527001 encode the plant enzyme Cytochrome P450 CYP79A2. Cytochromes p450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation.

CYP79A2 is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of the cyanogenic glycoside benzylglucosinolate. Oximes as the phenylacetaldoxime is generally toxic to fungi and is frequently used as chemical fungicides. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species.

BBa_K527001 codes for the same enzyme as BBa_K527000, but is submitted in an other plasmid due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix.

Notes

Mutated at a Pst1 restiction site (nucleotide 342)
No prefix and suffix.
User assembly ends.

Source

Institute of Plant Biology and Biotechnology
Faculty of LIFE Science
University of Copenhagen
Denmark

http://2011.igem.org/Team:DTU-Denmark-2

Sequence Annotation Location Component / Role(s)
start
stop
CYP79A2
mutation
1,3
1570,1572
1,1572
342,342
feature/start start_codon
feature/stop stop_codon
sequence_feature feature/misc
feature/mutation sequence_alteration
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K527001/1