Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eva Meier Carlsen
Date created: 2011-09-16 11:00:00
Date modified: 2015-05-08 01:12:35
Biosynthesis/Oximes/CYP79B1/Plug 'n' Play
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K527002_sequence (Version 1) |
Description
This BioBrick BBa_K527002 encode the plant enzyme Cytochrome P450 CYP79B1. Cytochromes p450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation.CYP79B1 is an N-hydroxylase converting L-Tryptophan into indole 3-acetaldoxime, the precursor of a cyanogenic glycoside . Oximes as the indole 3-acetaldoxime is generally toxic to fungi and is frequently used as a chemical fungicide. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species.
BBa_K527002 is submitted in the plasmid BBa_K678023 due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix.
Notes
Mutated at a Pst1 restiction site (nucleotide 342)No prefix and suffix.
User assembly ends.
Source
Institute of Plant Biology and BiotechnologyFaculty of LIFE Science
University of Copenhagen
Denmark
http://2011.igem.org/Team:DTU-Denmark-2