BBa_K531007

BBa_K531007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K531007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alexander Aaring

Date created: 2011-09-20 11:00:00

Date modified: 2015-05-08 01:12:36

P_rsaA constitutive promoter, esp optimized for Caulobacter, and rsaA

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K531007_sequence (Version 1)

Description

P_rsaA constitutive promoter from Caulobacter crescentus, esp, a suspected serine protease from Staphylococcus epidermidis, optimized for expression in Caulobacter crescentus, and the C-terminal secretion tag from rsaA.

Notes

Stop codon naturally occurring at the end of esp removed to ensure smooth expression of the fusion protein.

Source

P_rsaA and rsaA C-terminal secretion tag both come from the genome of Caulobacter crescentus; esp optimized for Caulobacter crescentus is a synthetically codon optimized copy of esp as it is found in Staphylococcus epidermidis.

Components

Access	Instance	Definition
public	BBa_K531006	BBa_K531006
public	BBa_K531001	BBa_K531001

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K531006	96,960	BBa_K531006
BBa_K531001	969,1328	BBa_K531001

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K531007/1

Member of these Collections

• iGEM 2018 Distribution Plate 6
• iGEM 2018 Distribution Plate 6 Well 20I
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM 2019 Distribution Plate 4
• iGEM 2019 Distribution Plate 4 Well 20I
• iGEM 2019 Part Library
• iGEM 2019 Plasmid pSB1C3
• iGEM Parts Registry