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BBa_K531009

BBa_K531009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K531009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alexander Aaring

Date created: 2011-09-22 11:00:00

Date modified: 2015-05-08 01:12:36

P_rsaA + Caulobacter optimized dspB + rsaA C-term

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K531009_sequence (Version 1)

Description

Constitutive promoter P_{rsaA with dspB codon optimized for Caulobacter crescentus and the C-terminal secretion tag of Caulobacter's S-layer associated protein, RsaA.}

Notes

Stop codon at the end of dspB removed to ensure expression of the rsaA C-terminal.

Source

P_{rsaA and rsaA C-terminal secretion tag both come from the genome of Caulobacter lab strain CB15N. IDT synthesized dspB so as to codon optimize it for expression in Caulobacter.}