Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alexander Aaring
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:36
Constitutive promoter and RBS BBa_K081005 + Caulobacter optimized esp + C-terminal sec
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K531011_sequence (Version 1) |
Description
Produces and secretes active Esp with intact C-terminal secretion tag from RsaA. Esp is a suspected serine protease that breaks up Staphylococcus aureus biofilms.Notes
Optimized esp does not have a stop codon to ensure expression of the fused secretion tag.Source
BBa_081005 was submitted to the registry by 2008 iGEM Team UNIV_Pavia, codon optimized esp was synthesized by Integrated DNA Technologies, rsaA C-terminal secretion tag was copied from Caulobacter lab strain CB15N.| Access | Instance | Definition |
| public public public | BBa_K081005 BBa_K531006 BBa_K531001 | BBa_K081005 BBa_K531006 BBa_K531001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K081005 BBa_K531006 BBa_K531001 | 1,58 67,931 940,1299 | BBa_K081005 BBa_K531006 BBa_K531001 |