Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ZHANG Yunxiao
Date created: 2011-09-28 11:00:00
Date modified: 2015-05-08 01:12:36
OmpA-SH3
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K533001_sequence (Version 1) |
Description
This part contains the N-terminal 180 amino acids of OmpA fused to SH3 domain in human Grb2 protein.The N-terminal of OmpA allows the moiety fused to it to be presented on to the outer membrane of E. Coli.
SH3 domain allows for binding to multi-proline peptide in human cells, which functions as a crucial adaptor module in signaling. In our part, this is specially for binding to the proteins for transportation by E. Coli.
All the coding sequence is under T7 promoter and lac repressor binding site. The parts is most efficiently presented onto the membrane when induced at 0.1mM IPTG at 18 centigrade in BL21 E. Coli strain.
Notes
High expression efficiency as well as inducible expression is most desirable when considering a coding sequence.Source
OmpA is derived from the genomic DNA of BL21 (DE3) E. Coli strain while SH3 is from human Grb2.| Sequence Annotation | Location | Component / Role(s) |
| lac operator T7 promoter OmpA SH3 | 20,44 1,18 87,632 633,815 | operator feature/operator feature/promoter promoter feature/cds CDS CDS feature/cds |