Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ZHANG Yunxiao
Date created: 2011-09-28 11:00:00
Date modified: 2015-05-08 01:12:36
OmpA-mCherry
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K533004_sequence (Version 1) |
Description
This is designed as the positive control for protein presentation on the outer membrane.Notes
His tag is inserted between OmpA and mCherry to facilitate detection after expression.Source
OmpA is cloned from genomic DNA of BL21(DE3) E. Coli strain.mCherry is from the Clontech plasmid pmCherry-C1.
| Sequence Annotation | Location | Component / Role(s) |
| T7 lacI rbs ATG OmpA His6Tag mCherry stop | 1,18 20,44 75,79 87,89 87,633 648,665 699,1409 1407,1409 | feature/promoter promoter operator feature/operator feature/rbs ribosome_entry_site start_codon feature/start CDS feature/cds feature/tag tag CDS feature/protein stop_codon feature/stop |