Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ZHANG Yunxiao
Date created: 2011-09-28 11:00:00
Date modified: 2015-05-08 01:12:36
OmpA-GFP
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K533005_sequence (Version 1) |
Description
GFP is presented outside E. Coli as a positive control for protein presentationNotes
His tag is inserted between OmpA and GFP for easier detection in Western Blotting.Source
OmpA is cloned from BL21(DE3) E. Coli strain. GFP is from Clontech plasmid pGFP-N1| Sequence Annotation | Location | Component / Role(s) |
| OmpA GFP rbs His Tag lacI T7 stop start | 87,633 696,1418 75,79 648,665 20,44 1,18 1416,1418 87,89 | feature/cds CDS feature/protein CDS feature/rbs ribosome_entry_site feature/tag tag feature/operator operator promoter feature/promoter stop_codon feature/stop start_codon feature/start |