atRS-ChZ

BBa_K537000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K537000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Natasia Kruger
Date created: 2011-07-18 11:00:00
Date modified: 2015-05-08 01:12:36

Atrazine Riboswitch-CheZ fusion



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K537000_sequence (Version 1)

Description

This DNA part will encode for an RNA riboswitch senstive to atrazine. The riboswitch, when no atrazine is present, prevents the exposure of the RBS. When atrazine is present, it binds to the riboswitch exposing the RBS and allows for the translation of the adjoining gene. In this case, the gene which will be expressed is CheZ, which a protein fundamental to bacterial movement.

Notes

The effectiveness of the atrazine riboswitch is determined by the ability of the RNA 'loop' to hide the RBS preventing translation. The sequence of bases within the riboswitch will determine the formation of the loop and its leakiness.

Source

The CheZ gene was isolated from genomic DNA using PCR where the forward primers in the reaction contained the sequence for the atrazine riboswitch.

Sequence Annotation Location Component / Role(s)
Atrazine riboswitch
C -> T
start
CheZ
1,88
55,55
86,88
86,727
sequence_feature feature/misc
feature/mutation sequence_alteration
feature/start start_codon
feature/cds CDS
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igem#sampleStatus
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synbiohub#ownedBy
user/james
 
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BBa_K537000/1