Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yang, Yi-Pei
Date created: 2011-09-21 11:00:00
Date modified: 2015-05-08 01:12:38
TetR repressible promoter (BBa_R0040)+ kivd (alpha-ketoisovalerate decarboxylase)+Ter (BBa_J61048)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K539741_sequence (Version 1) |
Description
This is the coding sequence of kivd from Lactococcus lactis subsp. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of α-keto acids derived from amino acid transamination. This is part of the isobutanol biosynthesis pathway. This part kivd is under TetR repressible promoter that constitutively ON and repressed by TetRcan. And terminator BBa_J61048 is included.Notes
It is a working biobrick.Source
Kivd is cloned form Lactococcus lactis subsp. lactis KF147| Access | Instance | Definition |
| public public public public | BBa_R0040 BBa_B0034 BBa_K539714 BBa_J61048 | p(tetR) BBa_B0034 BBa_K539714 BBa_J61048 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 BBa_B0034 BBa_K539714 BBa_J61048 | 1,54 63,74 81,1727 1736,1848 | p(tetR) BBa_B0034 BBa_K539714 BBa_J61048 |