BBa_K540001

BBa_K540001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K540001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mathilde Dumond
Date created: 2011-09-14 11:00:00
Date modified: 2015-07-14 12:34:23

rcn, cobalt-sensitive promoter



    • Details

    Types
    DnaRegion

    Roles
    Regulatory

    promoter

    Sequences BBa_K540001_sequence (Version 1)

    Description

    Rcn is a promoter that is activated by cobalt. A repressor is fixed to the promoter by default, and cobalt binds to this repressor, which activates the gene under control of this promoter.

    Rcn is also activated by nickel, but we have not characterized this aspect.

    Notes

    An internal iGEM restriction site had to be mutated by QuickChange mutagenesis.

    Source

    PCR from E.coli MC4100 genome.

    Sequence Annotation Location Component / Role(s)
    RcnR
    		1,279
    		feature/cds CDS

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K540001/1
     

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