Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mustafa Elitok
Date created: 2011-07-18 11:00:00
Date modified: 2015-05-08 01:12:38
Reflectin 1A (SacB) and LALF (SacB) protein for B.Subtilis
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K541024_sequence (Version 1) |
Description
Reflectin is a self-assembling protein that has the ability to reflect the sun light which depends on the thickness of the protein layer. Therefore, we thought that this ability could be used as a novel reporter for B.subtilis and E.coli.On the other hand, Lipopolysaccharide (LPS), or endotoxin, is the major mediator of septic shock, a serious complication of Gram-negative bacterial infections in humans. Molecules that bind LPS and neutralize its biological effects or enhance its clearance could have important clinical applications. Limulus anti-LPS factor (LALF) binds LPS tightly, and, in animal models, reduces mortality when administered before or after LPS challenge or bacterial infection. The wedge- shaped molecule has a striking charge distribution and amphipathicity that suggest how it can insert into membranes. The binding site for LPS probably involves an extended amphipathic loop, and it has been proposed that two mammalian LPS-binding proteins will have a similar loop. The amphipathic loop structure may be used in the design of molecules with therapeutic properties against septic shock.
Notes
Optimized for B.subtilisSource
LALF from Horseshoe crab, Reflectin from Cephalopod, promoter-RBS from B.subtilis| Sequence Annotation | Location | Component / Role(s) |
| BBa_K143053 BBa_K143012 Sigma A-35 Sigma A -10 BBa_K143021 rbs SacB LALF BBa_K541001 BBa_K143012 Sigma A-35 Sigma A -10 BBa_K143021 rbs Reflectin BBa_K541013 BBa_K541020 | 1,117 1,97 63,68 86,91 106,117 106,117 124,219 226,537 1,537 546,642 608,613 631,636 651,662 651,662 669,1523 669,1523 546,1523 | feature/BioBrick engineered_region feature/BioBrick engineered_region feature/promoter promoter promoter feature/promoter feature/BioBrick engineered_region feature/rbs ribosome_entry_site feature/tag tag feature/protein CDS feature/BioBrick engineered_region feature/BioBrick engineered_region promoter feature/promoter promoter feature/promoter feature/BioBrick engineered_region feature/rbs ribosome_entry_site feature/protein CDS engineered_region feature/BioBrick feature/BioBrick engineered_region |