Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cihan TAŞTAN
Date created: 2011-07-18 11:00:00
Date modified: 2015-05-08 01:12:38
Promoter Pveg, RBS spoVG and SacB signal peptide for B. subtilis
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K541501_sequence (Version 1) |
Description
Constitutive promoter veg(BBa_K143012) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis. These parts have been taken from 2008_Imperial_Collage. Pveg is a constitutive promoter that constitutively expresses the P43 protein in B. subtilis.SpoVG is an endogenous ribosome binding site from B. subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B. subtilis.
SacB is a signal peptide used in the Sec-SRP (secretory signal recognition particle) pathway by B. subtilis. Signal peptides are responsible for directing preproteins (secretory proteins with a signal peptide region attached) through an appropriate secretory pathway. In the case of the Sec-SRP signal peptide, they direct preproteins from the cytoplasm into the growth medium.
Notes
no any design considerationSource
All three basic components come from B.subtilis genome| Sequence Annotation | Location | Component / Role(s) |
| BBa_K143012 Sigma A-35 Sigma A -10 rbs BBa_K143021 BBa_K143053 SacB Signal tag | 1,97 63,68 86,91 106,117 106,117 1,117 124,219 | engineered_region feature/BioBrick feature/promoter promoter feature/promoter promoter ribosome_entry_site feature/rbs engineered_region feature/BioBrick feature/BioBrick engineered_region feature/tag tag |