Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Fazilet Guler
Date created: 2011-07-18 11:00:00
Date modified: 2015-05-08 01:12:38
Promoter Pveg, RBS spoVG and LipA signal peptide for B. subtilis
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K541502_sequence (Version 1) |
Description
Constitutive promoter veg(BBa_K143012) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis. These parts have been taken from 2008_Imperial_Collage. Pveg is a constitutive promoter that constitutively expresses the P43 protein in B. subtilis. SpoVG is an endogenous ribosome binding site from B. subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B. subtilis.LipA is a signal peptide from the B. subtilis genome. In general, signal peptides are responsible for directing preproteins (secretory proteins with a signal peptide region attached)through an appropriate secretory pathway[3]. LipA has been successfully used in the secretion of heterologous proteins such as cutinase by B. subtilis.
Notes
optimized for b.subtilisSource
b.subtilis| Sequence Annotation | Location | Component / Role(s) |
| Sigma A-35 Sigma A -10 BBa_K143012 rbs BBa_K143021 LipA signal peptide | 63,68 86,91 1,97 106,117 106,117 124,225 | promoter feature/promoter feature/promoter promoter engineered_region feature/BioBrick ribosome_entry_site feature/rbs feature/BioBrick engineered_region feature/tag tag |