BBa_K541546

BBa_K541546 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K541546

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Fazilet Guler

Date created: 2011-07-18 11:00:00

Date modified: 2015-05-08 01:12:38

IPTG inducible PlacI, RBS,TAT signal peptide and reflectin

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K541546_sequence (Version 1)

Description

Twin arginine signal peptide is an efficient protein export signal sequence of E. coli. Its promoter expresses more in the presence of IPTG.
Reflectin is a self-assembling protein that has the ability to reflect the sun light which depends on the thickness of the protein layer. Therefore, we thought that this ability could be used as a novel reporter for B.subtilis and E.coli.

Notes

optimized for e.coli

Source

cephalopod and e.coli

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
end of LacI coding region (inactive)	1,88	CDS, feature/cds
BBa_R0010	1,200	feature/BioBrick, engineered_region
CAP binding site	89,126	feature/binding, non_covalent_binding_site
-35	137,142	feature/promoter, promoter
-10	161,166	feature/promoter, promoter
LacI binding site	166,200	feature/binding, non_covalent_binding_site
start	173,173	feature/start, start_codon
BBa_B0034	209,220	feature/BioBrick, engineered_region
conserved	213,216	feature/conserved, conserved_region
TAT signalling peptide	227,455	tag, feature/tag
BBa_K541016	1,455	engineered_region, feature/BioBrick
Reflectin	462,1310	CDS, feature/protein
BBa_K541013	462,1310	engineered_region, feature/BioBrick

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K541546/1

Member of these Collections

• //chassis/prokaryote/ecoli
• iGEM Parts Registry