| Types | DnaRegion
|
| Roles | Composite
engineered_region
|
| Sequences | BBa_K546001_sequence (Version 1)
|
Description
The F2621 BioBrick device contains a lux pL regulated luxR repressor/activator and a lux pR promoter that is linked to an 3OC6HSL, an AHL autoinducer molecule, synthetase. The lux pL promoter yields a weak constitutive downstream expression. When LuxR and 3OC6HSL are produced, the lux pL promoter, however, will be repressed by a complex of the two. If 3OC6HSL is present in the cell, the autoinducer inactivation enzyme A (aiiA), with an LVA Tag, will inactivate the 3OC6HSL autoinducer molecules.
Notes
The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation there has been put an extra R0063 BioBrick part promoter in front of the F2621 BioBrick device.
Source
The lux p promoters originate from the quorum sensing ability of Vibrio fischeri.
