Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonathan Herrmann
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:40
CrtI Gene-Codon Optimized for Yeast
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K552003_sequence (Version 1) |
Description
This DNA sequence codes for the enzyme CrtI, also known as phytoene desaturase. CrtI converts pytoene to lycopene which is an important step in the carotenoid biosynthesis pathway in yeast. This sequence also includes a PGK promoter and an ADH1 terminator.Notes
While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence.Source
The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm| Sequence Annotation | Location | Component / Role(s) |
| PGK Promoter Start Codon CrtI Stop Codon ADH1 Terminator | 1,380 381,383 381,2129 2127,2129 2130,2258 | promoter feature/promoter feature/start start_codon feature/cds CDS feature/stop stop_codon sequence_feature feature/misc |