BBa_K552003

BBa_K552003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K552003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonathan Herrmann
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:40

CrtI Gene-Codon Optimized for Yeast



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K552003_sequence (Version 1)

Description

This DNA sequence codes for the enzyme CrtI, also known as phytoene desaturase. CrtI converts pytoene to lycopene which is an important step in the carotenoid biosynthesis pathway in yeast. This sequence also includes a PGK promoter and an ADH1 terminator.

Notes

While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence.

Source

The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm

Sequence Annotation Location Component / Role(s)
PGK Promoter
Start Codon
CrtI
Stop Codon
ADH1 Terminator
1,380
381,383
381,2129
2127,2129
2130,2258
promoter feature/promoter
feature/start start_codon
feature/cds CDS
feature/stop stop_codon
sequence_feature feature/misc
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K552003/1