BBa_K558001

BBa_K558001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K558001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jovanna Casas

Date created: 2011-08-24 11:00:00

Date modified: 2015-05-08 01:12:41

s70/PDC/ADH Ethanol Generator

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K558001_sequence (Version 1)

Description

This intermediate part (along with a promoter) was designed for ethanol production. The ethanol produced is to be used as an additive for biofuel.

The pyruvate decarboxylase gene converts pyruvate to acetaldehyde. Acetaldehyde is then converted to ethanol by alcohol dehydrogenase.

Notes

none

Source

The sequence for these genes is based on Zymomonas mobilis, a microorganism that naturally produces ethanol.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Part:BBa_J23101	1,36	promoter, feature/promoter
RBS	37,51	ribosome_entry_site, feature/rbs
PDC Coding Region	52,1757	feature/cds, CDS
2x Stop	1756,1761	feature/stop, stop_codon
NotI Cut Site	1764,1771	sequence_feature, feature/misc
BBa_B0014	2952,3047	engineered_region, feature/BioBrick
XhoI Cut Site	1772,1778	feature/misc, sequence_feature
4x Stop	2941,2952	stop_codon, feature/stop
start	52,54	feature/start, start_codon
Stuffer Region	1762,1791	sequence_feature, feature/misc
ADH Coding Region	1792,2940	feature/cds, CDS
start	1792,1794	start_codon, feature/start

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K558001/1

Member of these Collections

• iGEM Parts Registry